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MJ Research thermal cycler ptc200
Thermal Cycler Ptc200, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ptc200+thermal+cycler/ptc+100+thermocycler/10__5423_slash_ppj__oa__11__2024__0179-49-8-10
Average 90 stars, based on 1 article reviews
thermal cycler ptc200 - by Bioz Stars, 2026-09
90/100 stars

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Polymerase Chain Reaction:

Article Title: Body Remains Left by Bird Predators as a Reliable Source for Population Genetic Studies in the Great Capricorn Beetle Cerambyx cerdo , a Veteran Oak Specialist
Article Snippet: All of them were amplified in a single multiplex reaction, which was performed using the Multiplex PCR Kit (Qiagen, Hilden, Germany) according to the protocol recommended by the manufacturer in a volume of 10 μL (5 μL of 2 × QIAGEN Multiplex Master Mix, from 75 to 125 nM of each primer pair, 0.5 mg/mL of BSA, 10 ng of template DNA and ddH20 up to the total volume of 10 μL). .. The thermal PCR conditions were as follows: an initial incubation at 95 °C for 15 min; nine touchdown cycles: 94 °C for 30 s, 56 °C (−0.5 °C per cycle) for 1 min 30 s and 72 °C for 1 min; 24 cycles of 94 °C for 30 s, 52 °C for 1 min 30 s and 72 °C for 1 min; final elongation at 72 °C for 10 min. For amplification, we used PTC200 thermal cycler (MJ Research, Waltham, MA, USA). .. Separation of the amplified fragments was performed on an ABI PRISM 3130xl automated sequencer (Applied Biosystems, Foster City, CA, USA) using a LIZ 600 size marker (Applied Biosystems, Foster City CA, USA).

Article Title: Looking for hypoxia-fingerprint in multiple myeloma and malignant melanoma patients' transcriptomic data using a binominal distribution model
Article Snippet: .. The PCR was performed in MJ Research PTC200 Thermal Cycler. ..

Article Title: Incidence and Clinicopathological Features of Differentiated High-Grade Thyroid Carcinomas: An Institutional Experience.
Article Snippet: Differentiated high-grade thyroid carcinoma (DHGTC) is a new entity in the 2022 WHO classification.. We aimed to investigate the incidence and clinicopathological features of differentiated HG thyroid carcinoma (DHGTC) and compare the clinicopathological parameters of DHGTC, DTC without HG features, and poorly differentiated thyroid carcinoma (PDTC).. A total of 1069 DTCs including papillary thyroid carcinomas (PTCs) and follicular thyroid carcinomas (FTCs) were included in this study.

Incubation:

Article Title: Body Remains Left by Bird Predators as a Reliable Source for Population Genetic Studies in the Great Capricorn Beetle Cerambyx cerdo , a Veteran Oak Specialist
Article Snippet: All of them were amplified in a single multiplex reaction, which was performed using the Multiplex PCR Kit (Qiagen, Hilden, Germany) according to the protocol recommended by the manufacturer in a volume of 10 μL (5 μL of 2 × QIAGEN Multiplex Master Mix, from 75 to 125 nM of each primer pair, 0.5 mg/mL of BSA, 10 ng of template DNA and ddH20 up to the total volume of 10 μL). .. The thermal PCR conditions were as follows: an initial incubation at 95 °C for 15 min; nine touchdown cycles: 94 °C for 30 s, 56 °C (−0.5 °C per cycle) for 1 min 30 s and 72 °C for 1 min; 24 cycles of 94 °C for 30 s, 52 °C for 1 min 30 s and 72 °C for 1 min; final elongation at 72 °C for 10 min. For amplification, we used PTC200 thermal cycler (MJ Research, Waltham, MA, USA). .. Separation of the amplified fragments was performed on an ABI PRISM 3130xl automated sequencer (Applied Biosystems, Foster City, CA, USA) using a LIZ 600 size marker (Applied Biosystems, Foster City CA, USA).

Amplification:

Article Title: Body Remains Left by Bird Predators as a Reliable Source for Population Genetic Studies in the Great Capricorn Beetle Cerambyx cerdo , a Veteran Oak Specialist
Article Snippet: All of them were amplified in a single multiplex reaction, which was performed using the Multiplex PCR Kit (Qiagen, Hilden, Germany) according to the protocol recommended by the manufacturer in a volume of 10 μL (5 μL of 2 × QIAGEN Multiplex Master Mix, from 75 to 125 nM of each primer pair, 0.5 mg/mL of BSA, 10 ng of template DNA and ddH20 up to the total volume of 10 μL). .. The thermal PCR conditions were as follows: an initial incubation at 95 °C for 15 min; nine touchdown cycles: 94 °C for 30 s, 56 °C (−0.5 °C per cycle) for 1 min 30 s and 72 °C for 1 min; 24 cycles of 94 °C for 30 s, 52 °C for 1 min 30 s and 72 °C for 1 min; final elongation at 72 °C for 10 min. For amplification, we used PTC200 thermal cycler (MJ Research, Waltham, MA, USA). .. Separation of the amplified fragments was performed on an ABI PRISM 3130xl automated sequencer (Applied Biosystems, Foster City, CA, USA) using a LIZ 600 size marker (Applied Biosystems, Foster City CA, USA).

Article Title: Incidence and Clinicopathological Features of Differentiated High-Grade Thyroid Carcinomas: An Institutional Experience.
Article Snippet: Differentiated high-grade thyroid carcinoma (DHGTC) is a new entity in the 2022 WHO classification.. We aimed to investigate the incidence and clinicopathological features of differentiated HG thyroid carcinoma (DHGTC) and compare the clinicopathological parameters of DHGTC, DTC without HG features, and poorly differentiated thyroid carcinoma (PDTC).. A total of 1069 DTCs including papillary thyroid carcinomas (PTCs) and follicular thyroid carcinomas (FTCs) were included in this study.

Article Title: Induction of chronic lymphocytic leukemia-like disease in STYK1/NOK transgenic mice.
Article Snippet: .. After denaturation at 94 C for 5 min, the reaction mixture was amplified for 35 cycles at a condition of 94 C for 30s, 50 C for 30s, 72 C for 1min using a PTC200 Thermal Cycler (MJ Research). .. The following antibodies, each purchased from eBioscience, were used for flow cytometric analysis: PE anti-mouse CD5, FITC anti-mouse CD19.

Article Title: Gene Regulation and Global DNA Methylation Changes in White Spruce (Picea glauca) in Response to Copper Contaminations
Article Snippet: Plants require metals such as copper to survive, but an excess of metals can have phytotoxic effects.. The objectives of this study were to (1) analyze the expression of genes associated with copper resistance (RAN1 and MRP4) and (2) assess the effects of copper and potassium ions on global DNA methylation in white spruce (P. glauca).. Seedlings were treated with three concentrations of copper sulfate including 1312 mg/kg, 656 mg/kg, and 130 mg/kg.

other:

Article Title: Elimination of Tobacco rattle virus from viruliferous Paratrichodorus allius in greenhouse pot experiments through cultivation of castle russet
Article Snippet: Thermal cycling (PTC200 thermal cycler, MJ Research) consisted of 15 min at 50°C, 5 min at 94°C, then 30 cycles of 15 sec at 94°C, 1 min at 58°C, 30 sec at 72°C, and a final incubation for 5 min at 72°C.

Reverse Transcription:

Article Title: The Effects of Targeted Temperature Management on Oxygen-Glucose Deprivation/Reperfusion-Induced Injury and DAMP Release in Murine Primary Cardiomyocytes
Article Snippet: RNA concentration and purity were determined by spectrophotometric measurements at 260 and 280 nm using a Nanodrop 2000 (Nanodrop) and 1% agarose gel electrophoresis, respectively. .. CDNA was transcribed from 1000ng total RNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) and PTC200 Thermal Cycler (MJ Research). .. Expression of target genes and the endogenous control, gylcerinaldehyde-3-phosphate dehydrogenase (GAPDH), was assessed by real-time qPCR using the TaqMan® Gene Expression Assays (see Table 1) and StepOnePlusTM RealTime PCR System (Applied Biosystems) according to manufacturer’s recommendations.



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